Clinic Signification

Vaginitis is a common disease in obstetrics and gynecology outpatient department, It mostly caused by various pathogens infection. Domestic epidemiological research shows that the most common is various type of vaginitis patients in gynecological outpatient department, accounting for more than 30% of the total. The Clinical common includes  bacterial vaginitis (22% - 50% of female symptoms), fungal vaginitis (17% - 39%), trichomonal vaginitis (4% - 35%), senile vaginitis and young female vaginitis.

It can significantly improve diagnosis experience and effect by standard and precise treatment , In clinic, It can screen different pathogen by testing the vaginal secretion .It can use different treatment methods by different pathogen infection, which is helpful for early diagnosis and treatment of vaginal diseases, It can reduce the incidence of complications and the risk of related diseases. It is of great social value to protect women's health and improve their fertility.

background and principle

 
The Immunofluorescence staining is a new staining solution for rapid detection of vaginal microbial pathogens. It is stained by the combination for the reagent fluorescence dye labelled antibody and the vaginal microbial pathogen , It is showed strong fluorescence under the excitation of ultraviolet light. It observe the fluorescence intensity and morphology w
 by fluorescence microscope ,so that it can judge the existence of vaginal microbial pathogens

By the principle of the immunology and fluorescence staining ,The reagent cassette can mark various cell , bacteria,fungal,and trichomonas in the vaginal secretion,  It can observe at the the same which use double beam UV and B under the fluorescence microscope in one glass slide ,it is used for the fluorescence immunomorphological examination for vaginal microbial pathogens

Application

   
This reagent is a IVD(in -vitro diagnostic) reagent  which used for the special staining treatment
of vaginal secretion samples, It is used for the staining of white blood cells, epithelial cells, clue cells, fungi, bacteria, trichomonas in vaginal secretions, which can be easy for observation and judgment.


 Product Feature 
 

1. It can clearly provide fungus, trichomonas, clue cell basis
2.  It is more accurate than the humidity method for the mixed infection distinguish   It has more advantages in judging spores
3. It is no effect for trichomonad inactivation so that can  reduce missed diagnosis
4. It is more higher clinical coincidence rate than enzyme chemical method
5. It is more simple and rapid than  gram staining method
6. Comparison of diagnostic method of various pathogen

Fungal is the large group of the eukaryotes, it has significantly different from plants , animals and bacteria.The gungal cell wall contain chitin ,cellulose ,glucan, mannose etc , the lower fungal cell wall is mainly composed of cellulose , yeast is mainly composed to the glucan ,the higher fungal is mainly composed to the chitin


 
The fungal infection is meaning that one or more pathogenic fungal invade the body tissues , it can cause the disease by the fungal or its metabolites .The range is from superficial infection (such as skin infection , mucosal ulcers) to deep tissue infection (such as severe lung infection ,sepsis , or systemic diseases )

 There are about more than 400kinds of pathogenic fungal , the main pathogenic fungal are classified as follows:
 
Because of the lack of understanding of the biological characteristics of pathogenic fungal , In addtion, the variety of clinical manifestations of fungal infection, it is easy to miss diagnosis and misdiagnosis, cause the aggravation of the disease and even life-threatening, Therefore It need an effective and rapid early diagnosis method In clinic  

 Principle

The fluorescence brightener is one fluorescence dye,which has strong affinity with the chitin and cellulose of the fungal cell wall,It will emit bright blue fluorescence under the ultraviolet light (wavelength 340nm-380nm), it observe the morphology by the fluorescence microscope , so that it can fast detect the fungal

  Operation Progress


 Specification and clinical signification

1. Simple quick-low technical requirement for the test person,it can read result after 2 minutes ,it can be easily decide in clinic
2. High sensitivity --compare with the traditional direct microscopy method,high rate positive detection ,reduce the occurrence of false positive ,reduce the miss detection rate ,improve the accuracy of the test result

1. High identification --it has a clear fluorescence under the fluorescent microscope , clear image outline , easy to observe, reduce the error of the operating person initiative judgement
2. Wide application range :it can only detect certain fungal by the direct microscopy , but the fluorescence dye can be combination with all kinds of fungal ,then emit the fluorescence ,so it can all be detected of the shallow and deep infection fungal

Therefore , the fluorescence staining is a new rapid and effective method of testing fungal ,it not only provides the basis of rapid accurate fungal infection diseases,but also creates conditions for rapid diagnosis and rational drug use
l

 Interpretation of the result
Technology comparison of common fungal diagnosis  
 scope of application and specimen

 
The fungal immunofluorescence staining solution is mainly used for rapid tissue staining , it mainly stain for the fixation of formalin,paraffin-embedded tissue's fungal and the polysaccharides in the fungal cell wall
It is Suitable for samples of dander, nail polish, hair, sputum, body fluids and vaginal secretions

Collection method of common specimen 

1. Skin specimen:firstly disinfect the diseased position with 75% alcohol ,select the moving edge of the lesion , and scrape the epidermal dander with a blunt scalpel
2. Hair :detection of the brittle and loose hair from the root of the disease hair
3. Sputum:take the morning sputum, if take the expectoration,it must brush your teeth on an empty stomach to disinfect your mouth  
4. Vaginal secretions:use a long cotton sterilized swab, collect the white false membrane at the posterior vault of the vagina and cervix
5. Ophthalmic specimen :as for the keratitis specimen , please use the ophthalmic spoon scrape the fester and ulcer specimen under the operation microscope , as for the conjunctiva specimen , it is dipped directly with the wet swab in the normal saline

 Fluorescence microscope
With infinite optical system ,UV LED is the main fluorescence exciation light source ,it can bring you a clear full field vision ,high contrast fluorescence image , meanwhile , Abbe concentrator increase the visibility and contrast of specimen , excellent image and simple operation ,it can widely used in the biological research , medical inspection and other filed,it can fast detect the fungal
Specification and clinical signification

1. Simple quick-low technical requirement for the test person,it can read result after 2 minutes ,it can be easily decide in clinic
2. High sensitivity --compare with the traditional direct microscopy method,high rate positive detection ,reduce the occurrence of false positive ,reduce the miss detection rate ,improve the accuracy of the test result

1. High identification --it has a clear fluorescence under the fluorescent microscope , clear image outline , easy to observe, reduce the error of the operating person initiative judgement
2. Wide application range :it can only detect certain fungal by the direct microscopy , but the fluorescence dye can be combination with all kinds of fungal ,then emit the fluorescence ,so it can all be detected of the shallow and deep infection fungal

Therefore , the fluorescence staining is a new rapid and effective method of testing fungal ,it not only provides the basis of rapid accurate fungal infection diseases,but also creates conditions for rapid diagnosis and rational drug use
l

 Interpretation of the result  Fluorescence module



fluorescent module series products provides a new solution for laboratory fluorescence observation.With the LED fluorescence module, it is easy to convert a normal infinite optical  system microscope  into a modular, high efficiency  and easy-to-operate fluorescence microscope, enabling efficient fluorescence observation, do not need to purchase an expensive LED fluorescence microscope
the unique advantage